reporter cell line b16 bluetm ifn α β  (InvivoGen)

Journal: Biomedical journal

Article Title: Lidocaine reinforces the anti-inflammatory action of dexamethasone on myeloid and epithelial cells activated by inflammatory cytokines or SARS-CoV-2 infection.

doi: 10.1016/j.bj.2022.07.008

Figure Lengend Snippet: Fig. 3 Lidocaine in combination with dexamethasone has an additive modulatory effect different signaling pathways. TNFR signaling (A): THP1-XBlueTM-defMyD cells were stimulated ON with hTNFa and modulated or not with lidocaine and/or dexamethasone, and hIL-8 was measured in culture supernatants. IFNgR signaling (B): B16 IFNg SEAP reporter cells were stimulated ON with mIFNg in presence or not of different concentrations of lidocaine and/or dexamethasone, and SEAP activity was measured in supernatants. NF-kB signaling (C): HEK-hTLR4 SEAP reporter cells were stimulated ON with EcLPS and modulated or not with lidocaine and/or dexamethasone, and relative SEAP activity was measured in culture supernatants. Lidocaine and dexamethasone additively modulate poly I:C TLR3 signaling, but do not affect IFNAR signaling (DeE). B16 type I IFNs SEAP reporter cells were stimulated ON with poly I:C (D) or with supernatant from the same cell line previously stimulated with poly I:C (E) and modulated or not with lidocaine and/or dexamethasone. SEAP activity was measured in culture supernatants. * (p < 0.05), ** (p < 0.01), *** (p < 0.001), ****(p < 0.0001), a result significantly different from the stimulated unmodulated condition. # (p < 0.05), ## (p < 0.01), a result significantly different from its counterpart stimulated and modulated with only lidocaine at the same concentration. Data are represented as mean ± SEM of three replicates. Graphs are representative of at least two independent assays.

Article Snippet: B16 interferon gamma (IFNg) reporter cell line (InvivoGen®).

Techniques: Protein-Protein interactions, Activity Assay, Concentration Assay

Journal: Biomedical Journal

Article Title: Lidocaine reinforces the anti-inflammatory action of dexamethasone on myeloid and epithelial cells activated by inflammatory cytokines or SARS-CoV-2 infection

doi: 10.1016/j.bj.2022.07.008

Figure Lengend Snippet: Lidocaine in combination with Dexamethasone has an additive modulatory effect different signaling pathways. TNFR signaling (A): THP1-XBlueTM-defMyD cells were stimulated ON with hTNFα and modulated or not with Lidocaine and/or Dexamethasone, and hIL-8 was measured in culture supernatants . IFNγR signaling (B): B16 IFNγ SEAP reporter cells were stimulated ON with mIFNγ in presence or not of different concentrations of Lidocaine and/or Dexamethasone, and SEAP activity was measured in supernatants. NF-κB signaling (C) : HEK-hTLR4 SEAP reporter cells were stimulated ON with EcLPS and modulated or not with Lidocaine and/or Dexamethasone, and relative SEAP activity was measured in culture supernatants. Lidocaine and Dexamethasone additively modulate poly I:C TLR3 signaling, but do not affect IFNAR signaling (D-E). B16 type I IFNs SEAP reporter cells were stimulated ON with poly I:C (D) or with supernatant from the same cell lines previously stimulated with poly I:C (E) and modulated or not with Lidocaine and/or Dexamethasone. SEAP activity was measured in culture supernatants. * (p < 0.05), ** (p < 0.01), *** (p < 0.001), ****(p < 0.0001), a result significantly different from the stimulated unmodulated condition. # (p < 0.05), ## (p < 0.01), a result significantly different from its counterpart stimulated and modulated with only Lidocaine at the same concentration. Data are represented as mean ± SEM of three replicates. Graphs are representative of at least two independent assays.

Article Snippet: B16 IFNγ reporter cell line (InvivoGen®).

Techniques: Activity Assay, Concentration Assay

Journal: Biomedical Journal

Article Title: Lidocaine reinforces the anti-inflammatory action of dexamethasone on myeloid and epithelial cells activated by inflammatory cytokines or SARS-CoV-2 infection

doi: 10.1016/j.bj.2022.07.008

Figure Lengend Snippet: Lidocaine in combination with dexamethasone has an additive modulatory effect different signaling pathways. TNFR signaling (A): THP1-XBlueTM-defMyD cells were stimulated ON with hTNFα and modulated or not with lidocaine and/or dexamethasone, and hIL-8 was measured in culture supernatants . IFNγR signaling (B): B16 IFNγ SEAP reporter cells were stimulated ON with mIFNγ in presence or not of different concentrations of lidocaine and/or dexamethasone, and SEAP activity was measured in supernatants. NF-κB signaling (C) : HEK-hTLR4 SEAP reporter cells were stimulated ON with EcLPS and modulated or not with lidocaine and/or dexamethasone, and relative SEAP activity was measured in culture supernatants. Lidocaine and dexamethasone additively modulate poly I:C TLR3 signaling, but do not affect IFNAR signaling (D – E). B16 type I IFNs SEAP reporter cells were stimulated ON with poly I:C (D) or with supernatant from the same cell line previously stimulated with poly I:C (E) and modulated or not with lidocaine and/or dexamethasone. SEAP activity was measured in culture supernatants. ∗ ( p < 0.05), ∗∗ ( p < 0.01), ∗∗∗ ( p < 0.001), ∗∗∗∗( p < 0.0001), a result significantly different from the stimulated unmodulated condition. # ( p < 0.05), ## ( p < 0.01), a result significantly different from its counterpart stimulated and modulated with only lidocaine at the same concentration. Data are represented as mean ± SEM of three replicates. Graphs are representative of at least two independent assays.

Article Snippet: B16 interferon gamma (IFNγ) reporter cell line (InvivoGen®) .

Techniques: Activity Assay, Concentration Assay

Journal: Cell

Article Title: M. tuberculosis Reprograms Hematopoietic Stem Cells to Limit Myelopoiesis and Impair Trained Immunity

doi: 10.1016/j.cell.2020.09.062

Figure Lengend Snippet:

Article Snippet: Secretion of total active IFN-I (both IFN-α and IFN-β) in BM supernatants was assessed using B16-blue IFNα/β reporter cell line (InvivoGen), according to the specifications of the manufacturer.

Techniques: Staining, Virus, Recombinant, Lysis, Isolation, Expressing, Software, Luminex